Can anyone tell me what combination of phytohormones and medium composition suit best for in vitro flowering of bulbous and cormous species?
You may try these PGR combinations for in vitro flowering. These combinations work well with many bulbous and ornamental plants
1. TDZ (0.2-0.5 mg/l) in combination with NAA (0.1- 0.25mg/l)
2. BAP alone (0.25- 2 mg/l) or in combination IAA or NAA (0.1- 0.5mg/l)
Kind Regards
Shiwani Kaushal
As we know flowering in in vivo condition depends on PGR like gibberellins + anthesin (hypothetical), thus application of GA in combination with an auxin or cytokinin will work. Combination and conc. must be ascertained through experimentation.
Good luck
Quite interesting. I obtained low-frequency (>4%) in vitro flowering in regenerated shoots of the tree species Melia volkensii (Meliaceae) with 1/2 MS, 3/4MS and MS supplemented with 0.05 mg/l TDZ. No experience with bulbous plants but will go with Kaushal's answer.
G-5 media supplemented with 0.5 mgL-1 BAP, 0.1 mgL-1 NAA & 0.5 mgL-1 2, 4-D using half ovary as an explant
Hi, Asif, Do you want to induce flowering transition or get "mature" flowers?
For the induction, you need to create generative meristem, ea. create a local high auxin accumulation. Yes, BAP, TDZ or GSH are good treatment for induction, but high concentration may prevent further flowers development. High sucrose concentration is also essential. So, you have to use 2 step treatment: high sucrose + BAP (TDZ, kinetin) + high light, better on the optimal medium.
And, as soon as flowers have been induced, transfer to normal, but optimal medium.
Best wishes!
Thank you all for yours suggestions.
Dear Pasternak,
I want to induce flower first and then will acclimatize it but first my aim is to in vitro induce flower. You are right that making double the amount of sucrose give good result as it has been reported in orchids and some other plants. In literature i have found that some have used coconut water with TDZ + GA3 with different photoperiods. Do you think is there any need of auxins if you have to supplement Coconut water + TDZ + GA3 + low to high conc. of sucrose to the medium
Regards
Asif Khan
Did any one know TDZ available in India??? If anybody knows that. Kindly drop me details to [email protected]
you will find TDZ on this link given below.
http://www.sigmaaldrich.com/catalog/product/sigma/p6186?lang=en®ion=IN
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