What is the best coating to differentiate mES into neurons?

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Mireia Moreno Estelles

Thank you Marcus.

Overall, can you tell me what coating do you use?

Cherif Badja

Hi,

The two are working well.

I am deriving neurons from hiPSCs and I am using Poly-D-Lysine/Laminin (10ug/ml each) and it works good.

However coating with Ploy-D-Lysine/Fibronectin (10ug/ml each) can works also, but for better results I prefer to use Laminin.

Good luck