I am trying to extract membrane proteins from rat's organs with high concentration, which buffer you recommend me?
Need fruitful guidance
07 April 2024 3,275 1 View
Dear ResearchGate Community, I am currently conducting research focused on flood extent analysis and calibration of a Storm Water Management Model (SWMM). As part of this study, I am interested in...
14 March 2024 2,508 0 View
What is the best way to find a bunch of email addresses related to academics working on "Plant Endophytes"?
07 March 2024 5,395 1 View
all datasheet information are based on 1 sun irradiation (1000 W/m2), in room condition, or with LED lightening is it possible to provide such this irradiation? for the PV cells, assume we make a...
06 November 2023 5,737 1 View
Hello everyone, I would like to purchase male microneedles. Would you please introduce me a company which make them? Thanks.
13 September 2023 6,152 3 View
Hello everyone, I conducted a native gel electrophoresis experiment for my protein, which has a pI of around 8.5. I used the protocol provided in this link:...
06 September 2023 3,022 2 View
"I have untreated cells as a control group and three concentrations of a drug. Each group consists of three replicate wells, and I conducted the same experiment three times. I want to compare the...
15 May 2023 7,735 1 View
I am encountering issues with building DSM/POPE/POPC/Cholesterol membrane . My job id (if I read my files correctly) is 8388718807. The error states: "CHARMM was terminated abnormally. Please...
11 May 2023 5,135 1 View
I have made a membrane + protein system in Charmm gui can you give me methods to "how to do analysis on membrane protein computationaly?"
01 March 2023 5,063 4 View
Hello, I tried different methods of making refolding buffer including cystine, I dissolve it in HCL first but then when I raise the pH to 8 I can see it solidifying after raising the pH. how can I...
17 February 2023 2,564 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View