Mehr Kimia

4 Questions 7 Answers 0 Followers

Questions related from Mehr Kimia

What is the best buffer for protein extraction from animal tissue?

I am trying to extract membrane proteins from rat's organs with high concentration, which buffer you recommend me?

11 July 2013 4,540 0 View

How can I take the endometrium of uterus from Rat?

I want to quantify a protein in rat uterine endometrium. As this protein may present in the other parts of uterus, this section should be separated from uterus, but I don't know how. Can any one...

04 May 2013 6,358 2 View

How can I remove the staining from the background in IHC?

I am doing IHC using an ABC staining kit (centacruz), concentration of primary and secondary antibody is optimized to detect the desired protein, incubate DAB 30 min, counterstain 5 second, but...

09 March 2013 3,735 2 View

What is the best way to increase the concentration of proteins?

I extracted total proteins from tissue to do western blot. Some of my samples are in low concentration. Since I need to run equal proteins for SDS page, I want to increase the concentration of...

09 February 2013 2,001 25 View