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Questions related from Cory Antonio Ocasio
How many nucleotides can be placed between the overlap and annealing section of the final reverse primer for NEB assembly (Gibson assembly)? P/T2A ok? This would be the last reverse primer that...
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Hello Everyone, I"m looking into independently learning PEAKS and Perseus on my free time and wondering if anyone can recommend a tutorial or demo?
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In the past, I have obtained varying results with Phusion, but have been told there are other polymerases that can deal with the GC richness of some regions in genomic DNA. Do I really need to buy...
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Initially, our paper was reviewed by three people, and then a 4th. 3 suggested publication with 2 suggesting only minor edits; one was strongly opposed. After the rebuttal, the same three clearly...
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I would like to stably express these genes in cancer cells while removing expression of wildtype protein.
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The plasmid is siRNA resistant, and I'd like to show a rescue in cell cycle phenotype, so it is important to have plasmid expressed first or very soon after si tfx.
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