Cory Antonio Ocasio

6 Questions 19 Answers 0 Followers

Questions related from Cory Antonio Ocasio

What is the maximum length of the spacer region in a Gibson assembly primer?

How many nucleotides can be placed between the overlap and annealing section of the final reverse primer for NEB assembly (Gibson assembly)? P/T2A ok? This would be the last reverse primer that...

11 November 2017 7,298 4 View

Recommendations for independently learning how to use PEAKS/Perseus?

Hello Everyone, I"m looking into independently learning PEAKS and Perseus on my free time and wondering if anyone can recommend a tutorial or demo?

11 November 2017 7,390 0 View

Hello there, I have a question about performing PCR on genomic preps to validate targeting of my construct using CRISPR?

In the past, I have obtained varying results with Phusion, but have been told there are other polymerases that can deal with the GC richness of some regions in genomic DNA. Do I really need to buy...

08 August 2016 3,639 9 View

My question relates to the process of publishing with the Journal of Medicinal Chemistry; particularly a way to address concern over unfair treatment?

Initially, our paper was reviewed by three people, and then a 4th. 3 suggested publication with 2 suggesting only minor edits; one was strongly opposed. After the rebuttal, the same three clearly...

07 July 2016 2,285 9 View

What is the best technique to use for genome modification, particularly to modify and stably express two genes while deleting the WT gene?

I would like to stably express these genes in cancer cells while removing expression of wildtype protein.

11 November 2013 4,622 4 View

What is the best way to introduce a plasmid and siRNA at the same time if possible?

The plasmid is siRNA resistant, and I'd like to show a rescue in cell cycle phenotype, so it is important to have plasmid expressed first or very soon after si tfx.

11 November 2013 3,050 11 View