Shadid Uz Zaman

12 Questions 7 Answers 0 Followers

Questions related from Shadid Uz Zaman

Is reporting Wald Statistic Very Important when reporting results of multinomial logistic regression analysis?

I am performing statistical analysis on a data where I am trying to see observe the impact of factors like sex, area of residence and literacy on outcome variables. I don't have any SPSS...

04 December 2020 8,600 9 View

Is there any formulas or equations to calculate the dose of a drug for transdermal and topical patch preparation?

I want to prepare a topical and a transdermal patch system of a certain drug which is only available orally at the moment. How can I calculate the dose of the drug for the patch system? For...

28 May 2019 5,240 5 View

What is Flatness of transdermal patches?

I am about to prepare a formulation of transdermal patch. So I was going through various journals. Many of these a term called "flatness" to evaluate the prepared patches. But I am not clear...

27 May 2018 5,961 2 View

Is it possible to develop a rudimentary method of estimating gene expression using Normal PCR?

I have no expertise with PCR at all, but I do understand the principle behind PCR, qPCR and RT-PCR. I want to estimate the expression of certain gene in bacteria. But right now, I only need a...

04 December 2017 3,218 5 View

What is the best aqueous solvent to dissolve Reserpine?

I want to prepare an aqueous solution of Reserpine to test it's MIC. I've tried to use 8% DMSO as the solvent (which will give me final conc. of 2% DMSO during the assay) but Reserpine...

01 December 2017 6,615 2 View

Questions about Ethidium bromide-agar cartwheel method?

I want to evaluate efflux pump activity using the Ethidium bromide-agar cartwheel method. But in this method, I will have to prepare agar plates containing Ethidium bromide and inoculate bacteria....

11 October 2017 519 6 View

How to identify E. coli conclusively?

I currently have 16 multidrug resistant strains of E. coli (supposedly). But I need to ensure they ARE actually E. coli. I have been directed to IMViC test which doesn't seem conclusive or...

30 September 2017 6,321 7 View

Can I sterilize Tween 80 by autoclaving?

I am trying to observe antimicrobial activity of Tween 80 on certain clinical isolates of E. coli and P. aeruginosa that I have collected. I need to sterilize Tween for this. However, right now my...

20 August 2017 1,722 39 View

Can I sterilize graduated glass pipettes by DHS or Autoclave without damaging them?

I am worried that if I sterilize glass (pyrex) pipettes by DHS or Autoclave the graduations in the pipettes will no longer be accurate. However, most protocols does allow DHS or Autoclave. So, is...

13 June 2017 7,986 2 View

Glycerol or Paraffin oil, which is better for bacteria preservation?

I am exploring options to preserve bacteria namely, Staph. aureus, E. coli, P. aeruginosa and Salmonella typhi. I want to be able to preserve them for a period of 1 year and will be using them for...

07 June 2017 2,274 8 View

What are the ATCC strains with good/strong efflux pump activity?

I am working on a research that involves working with strains known to be over-producers of various classes of efflux pumps. I have seen that most of the research involving efflux pump...

08 May 2017 2,035 3 View

Is it possible to fractionate small peptides (<50AA) using analytical HPLC?

I am currently developing a research protocol involving antimicrobial peptides. I have searched various journal articles, and they describe that to purify these peptides I need to have sep-pak...

13 January 2017 6,038 3 View