What is the best approach for eliminating junk sequences?

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How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Does anyone know why I would have unexplained emission overlap for flow cytometry?

Hello, I am new to flow cytometry, and was just using a sample cell population to train with the technician. The cells were PAC-1 cells split into four groups. Unstained, transiently transfected...

28 January 2021 969 2 View

Does anyone know of a library for R that includes phi?

I'm looking to produce some phi correlations and I'd rather not do it manually, but I can't find a library for R that includes phi.

18 January 2021 2,808 9 View

In SEM, how to interpret standardised regression weights and their significance?

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31 December 2020 7,604 6 View

Does anyone know how to add custom text to a violin plot in r, ggpubr made using the ggviolin line?

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26 December 2020 4,480 4 View

How do I interpret these correlations and p-values?

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17 December 2020 9,825 4 View

Is it possible to fuse Deep Learning Net and Clustering Algotrithm?

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Are there models trained+evaluated on multiple datasets?

Generalization of COVID-19 models is a very serious challenge. I'm trying to find a model (ct scans or x-rays) that was trained and evaluated on multiple datasets. It would be particular...

03 December 2020 8,863 4 View

Are there models trained on both x-ray and ct scans for COVID-19 prediction?

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When examining a series with different numbers of episodes per series, should I treat it as single sample, use harmonic mean or some other approach?

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How to compare two groups with only two measurements?

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03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

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Do we need to write protocol for a second study of a systematic review that has been registered and published?

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03 March 2021 8,920 3 View

How to increase a model accuracy ?

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03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

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In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

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03 March 2021 8,066 4 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

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02 March 2021 4,362 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Polpass Arul Jose

Which technique you employed for sequencing? classic sequencing or NGS?

Alex K. Gaithuma

I used illumina system Miseq sequencing

Xabier Vázquez-Campos

Both mothur and QIIME have standard protocols for the analysis of 16S amplicon sequencing. If using this software is much of a pain for you, contact somebody with experience in the analysis of this kind of sequencing data.

I would be glad to collaborate if you need so.

http://www.mothur.org/wiki/MiSeq_SOP