Alex K. Gaithuma

3 Questions 6 Answers 0 Followers

Questions related from Alex K. Gaithuma

Why do I get multiple bands when I produce recombinant protein?

After production of recombinant protein using the Pichia pastoris system, I always get two bands (figA and B) of my target protein. This time I got three bands figC) after purification using...

18 August 2021 571 8 View

What is the best approach for eliminating junk sequences?

I have sequenced rRNA PCR amplicons from many samples, the target of the PCR primers having being the rRNA region. I need to distinguish between and identify the different species from the...

25 April 2016 4,888 3 View

Does tagging primers for NGS sequencing reduce their specificity?

I carried out PCR with ITS region specific primers on Tsetse fly derived DNA samples and got some bands as shown in the image shown....when i repeated the PCR with tagged primer for sequencing, I...

19 January 2016 1,263 3 View