Can you recommend an antibody for detection of M. tuberculosis H37Rv by fluorescence microscopy? I've tried a couple of them with no success; the background is so high. I'm looking for intra-macrophage bacteria. .
One could use monoclonal antibodies (MAbs) to M. tuberculosis. However, the monoclonal antibody has to be labelled with FITC. MAb against 38kDa will be of help.
Ivanyi J, Morris JA, Keen M. Studies with monoclonal antibodies: In Macario & Conway de Macario, eds. Monoclonal antibodies against bacteria. Acad Press Inc NY (1985) 1: 59-90.