I have been trying to acquire 2P images during awake and sleep states, however according to the EEG recordings, the mice are not falling asleep. I have recorded around 2-3 h but nothing happens. Any suggestions on what I can do to make it more "comfortable" for the mice to fall asleep while recording?
Fernanda Juarez
To test your system, you could start by doing a total sleep deprivation and then recording to ensure that the mice had sleep rebound and show the different sleep stages. Once you evaluate your system you could probably remove the sleep deprivation variable.
Otherwise, you need the mice to be completely habituated to the registration cages. You could do this by placing the mice in the recording conditions at least 7 days before your test day.
Hola, yo haría lo que Fernanda recomienda. Privar de sueño para que cuando haga el registro me asegure de que está dormido. Tambien puedes intentar inducirlo farmacológicamente. O habituar a los animales a los aparatos, tal vez acostumbrarlos a dormir con algo que simule tus aparatos del día de registro.
Saludos