Dear MD Nazmul Islam!

About miR-21:

microRNA 21 also known as hsa-mir-21 or miRNA21 is a mammalian microRNA that is encoded by the MIR21 gene.MIRN21 was one of the first mammalian microRNAs identified. The mature miR-21 sequence is strongly conserved throughout evolution. The human microRNA-21 gene is located on plus strand of chromosome 17q23.2 (55273409–55273480) within a coding gene TMEM49 (also called vacuole membrane protein). Despite being located in intronic regions of a coding gene in the direction of transcription, it has its own promoter regions and forms a ~3433-nt long primary transcript of miR-21 (known as pri-miR-21) which is independently transcribed. The stem–loop precursor of miR-21(pre-miR-21) resides between nucleotides 2445 and 2516 of pri-miR-21.

17q23.1  [Link to chromosome band 17q23]Location_base_pairStarts at 59841266 and ends at 59841337 bp from pter ( according to hg19-Feb_2009)  [Mapping MIR21.png]Local_orderBased on Mapviewer, genes flanking MIRN21 oriented from centromere to telomere on 17q23 are:

• TMEM49, transmembrane protein 49, 17q23.1 .
• MIRN21, microRNA 21, 17q23.1 .
• TUBD1, tubulin, delta 1, 17q23.1 .
• LOC729565 ,similar to NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa, 17q23.1 .
• RPS6KB1, ribosomal protein S6 kinase, 70kDa, polypeptide 1, 17q23.1 .
• I think that this is enough for begining. Sincerely yours! Dr. Bratko Filipič Email: [email protected]

calculate it from the sequence :-)

carfull, take the mature form and not the pri-miR-21 or pre-miR-21 )