I am looking for indication regarding the number of cells used for siRNA transfection. I have read papers that just "miss" this little detail. Some companies' technology notes state that you can use "150000 cells for transfection with 250 pmols of siRNA" for various types of cells. Has anyone experience in pancreatic beta cells and siRNA and willing to share the approx. number of cells he/she used in his/her siRNA experiments? Thank you in advance for any help!
I haven't tried pancreatic cells, but regular ratio is 0,5-2 pmol of siRNA per 1'000 cells. Higher amount of siRNA might produce off-target effects, lower conc. - low efficiency.
Thank you so much, Andrey! We need hands-on experience as papers are usually reluctant in this details! Have a nice day,
Monica
This may help you, Delivery of Antiviral siRNA with Gold Nanoparticle Against Dengue Virus
http://aquila.usm.edu/cgi/viewcontent.cgi?article=1111&context=honors_theses
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