We need to dissolve the drug and chitosan completely and already heard that DMSO, acetic acid and ascorbic acid can be used as a solvent for dissolving. We tried to dissolve the drug in 0.1% DMSO but it is not completely dissolving. If we want to use ascorbic acid as a solvent, what maximum concentration of ascorbic acid can be used for dissolving? Is ascorbic acid toxic to the cells at that particular concentration? It would be greatly helpful if someone clarifies this with reference.
That's weird, because from what I know ascorbic acid, unlike DMSO and acetic acid, is in solid form, perhaps what you mean is ascorbic acid solution? Second, regarding to toxicity, I don't know what would happen when you give ascorbic acid to cell lines. But I do know that there is no known lethal dose for human, I only heard that 10,000-50,000 mg of ascorbic acid a day to adult human may cause diarrhea.
Also I would like to clarify something, which one is failed to dissolve? Is it chitosan? Or the drug? Or both of them?
Regards,
Radif
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