I have the plot profile in imageJ and I saved the data (Distance_(microns) & Gray_Value) for the analysis. Could you please suggest to me how to do quantification with this?
This begs the question : what exactly is it that you want to quantify ?
But from my window, the quick answers would be :
on the GL histogram of your values : do the usual statistics, easy in ImageJ using the histogram & measurements option
consider the GL profile as you would a roughness profile and you can then compute all sorts of "GL roughness" parameters (lots of information around on the web on Roughness Parameters)
To my knowledge, there exist only a few plugins for roughness work on ImageJ, such as :
My inclination is generally to export the profile and do the computation outside ImageJ (Excel is sufficient for the most common parameters but I use MatLab or Mountains for topography computation)