What is the alternative to ammoniium acetate mention in DNA isolation protocol of Doyle &Doyle, 1991?

11 July 2024 1 1K Report

In the Doyle and Doyle, 1991 protocol for plant DNA isolation, requirement of ammonium acetate is mentioned. Is there any alternative to it?

Malcolm Nobre

Hello Shivansh Negi,

DNA is precipitated with either ethanol or isopropanol. Precipitation is usually

performed in the presence of salt such as ammonium acetate.

As an alternative, you may use sodium acetate instead of ammonium acetate.

Best.

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