Dear all,
I find a paper using Snapback primer. I do not understand the function of snapback tail?
What is snapback primer? When do we use snapback primer? and why we use that?
Thank you.
Snap back primers are primers with tails that hybridise back on themselves creating a hairpin: If that happens the primer is taken out faction
Thus if you have a WT sequence mixed with a mutant sequence in a mixed genotype population the WT sequence will be selectively amplified as there primer is able to fully hybridise to target allowing specific and efficient PCR whereas a SNP in target sequence will preclude efficient binding and thus allow the tail to snap back on itself, preventing PCR
I hope this helps
Snapback primer consists of unlabeled probe attached to the 5′-end of PCR primers without any modifications. A 2-bp mismatch at the 5′-end of the snapback primer may also be included to prevent 3′-end extension of the minor hairpin that may form from the full-length single strand that includes the limited primer.
You'll find the full demonstration in the following article :
Article Snapback primer mediated clamping PCR for detection of EGFR ...
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