Photoautotrophic tissue culture is defined as "micropropagation without sugar in the culture medium, in which the growth or accumulation of carbohydrates of cultures is dependent fully upon photosynthesis and inorganic nutrient uptake". There are multiple advantages to using this form of propagation, because this system actively encourages plant growth. Namely, there are lower contamination rates due to the lack of sugar in the growing medium, and more catering to plants that aren't able to be successfully multiplied by conventional means. Many plants have trouble being propagated to due physiological or morphological inhibitions that make root formation difficult from cuttings. And after the plantlet develops in vitro, then the ex-planting process can often be very stressful and result in plant death, which isn't a problem for pre-acclimated plantlets grown via photoautotrophic tissue culture (In this context, pre-acclimated refers to plantlets that have developed a cuticle and have been cultivated in an open air system as opposed to the closed system seen in typical operations).

https://en.wikipedia.org/wiki/Photoautotropic_tissue_culture

Photoautotrophic (sugar-free medium) micropropagation was introduced more than 20 years ago and is still a developing technology with high potential. This chapter reviews the research and development on photoautotrophic micropropagation (PAM), focusing on features of the in vitro environment for promoting in vitro plant growth. First, the concept of PAM and methods of improving the in vitro environment for…

Photoautotrophic Micropropagation. Available from: https://www.researchgate.net/publication/286927104_Photoautotrophic_Micropropagation [accessed May 28, 2017].

https://www.researchgate.net/publication/286927104_Photoautotrophic_Micropropagation

Chapter Photoautotrophic Micropropagation

it is great technology instead sugar containing mediums bcz its adaptive, safe, secure (less incidence of infection of pests-disease)

Chlorophyllous explants, shoots, and plantlets in vitro have high photosynthetic ability to develop photoautotrophy, but their photosynthetic activity is restricted largely by the low CO2 concentration in the vessel during the photoperiod and in part by the presence of sugar in the medium. The growth of plantlets in vitro is often greater under photoautotrophic conditions than under heterotrophic conditions, provided that the in vitro environment is properly controlled for promoting photosynthesis. u can see attached pdf for additional query 

Thanks for everyone

Please have a look on this 

http://www.sciencedirect.com/science/article/pii/S0921042301800897

Regards

Photoautotrophic micropropagation is a micropropagation without exogenous organic components such as; sugar, vitamins, etc. in the medium and the control of the in vitro environment  ( CO2 concentration, photon flux, relative humidity, and air speed) in the culture vessel. Photoautotrophic micropropagation reduce the cost of production and improve quality of planters.

Photoautotrophic micropropagation  is defined as the in vitro propagation of plants in a  sugar free medium.  It is also described as photosynthetic micropropagation or  inorganic micropropagation . Consult  the book Plant Tissue Culture Basic and Applied, 2016 published by Platinum Publisher, Kolkata, India

Photoautotrophic tissue culture is defined as "micropropagation without sugar in the culture medium, in which the growth or accumulation of carbohydrates of cultures is dependent fully upon photosynthesis and inorganic nutrient uptake.

There are multiple advantages to using this form of propagation, because this system actively encourages plant growth. Namely, there are lower contamination rates due to the lack of sugar in the growing medium, and more catering to plants that aren't able to be successfully multiplied by conventional means. Many plants have trouble being propagated to due physiological or morphological inhibitions that make root formation difficult from cuttings. And after the plantlet develops in vitro, then the ex-planting process can often be very stressful and result in plant death, which isn't a problem for pre-acclimated plantlets grown via photoautotrophic tissue culture (In this context, pre-acclimated refers to plantlets that have developed a cuticle and have been cultivated in an open air system as opposed to the closed system seen in typical operations).

Please check the following links for more details

http://www.arccjournals.com/uploads/articles/ar242009.pdf

http://www.aff.org.au/cross_banksia_final.pdf