How to separate host DNA from microbial DNA?

Hello People I am extracting host DNA from fecal samples. However, feces contains lot of microbes and their DNA too gets extracted during DNA extraction procedure. I want to get rid/minimise...

05 June 2019 6,544 6 View

Unable to download r8s from its website for divergence time estimate.

02 March 2016 9,891 1 View

How to convert excel format into mega format?

So is it possible to convert an excel file into mega format so that it can be opened in MEGA for alignment and analysis?

07 August 2014 1,290 3 View

How can I import/open a nexus (*.nxs) file into MEGA 5.2?

I have nexus file (*.nxs) which contains a lot of taxa and the sequence length is also very long, so I cannot keep copy pasting it as it will take a lot of time. I want to know if there is any...

05 June 2014 5,766 6 View

Why to study phylogenetics? What's its use?

I work on the phylogeny of one of the mammal groups but sometimes I wonder, why exactly am I doing this? What's the point in studying phylogenetics? Is there any direct benefits of my study for...

31 December 2013 9,762 0 View

What is the best way for calculating phylogenetic diversity?

I need to calculate phylogenetic diversity (PD) of many different areas. I found some ways to calculate it, but couldn't decide on which way is better. If anybody has any idea about it please let...

09 October 2013 2,861 6 View

Which Taq should be used while working with DNA extracted from fecal samples?

I am trying to amplify mitochondrial as well as nuclear DNA from fecal samples but the success rate is very, very low. The reactions are failing miserably. I would like to know what kind of Taq...

07 August 2013 4,034 6 View

Why use a non-coding nuclear marker in phylogenetic and evolutionary studies?

Whats the difference when we use a coding nuclear marker versus the non-coding nuclear marker? What difference does it make to the results? And how do we know when to use a nuclear coding region...

05 June 2013 4,648 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View

How to do mono formylation of N,N-diethyl-3-methoxybenzamide?

I have attempted the formylation of the above mentioned compound using a very well reported protocol using secondary Butyl Lithium and TMEDA at - 78 degrees Celsius. I have done it quite a few...

08 February 2021 2,793 2 View

Can anyone recommend me a NMR Handbook?

Hi, I've been searching for a NMR Handbook with 29Si chemical shifts information but I can't find one. Also I've tried to find an online database with that kind of info but I have no lucky. Can...

02 February 2021 307 3 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

RNA extraction from exocrine pancreas?

Hi everyone ! Does anyone have any experience in extracting RNA from exocrine pancreas (which is very high in RNAse)? The pancreas is frozen tissue. I wonder if RNAse is also high in the nucleus,...

31 January 2021 390 2 View

What is chloroplast capture through introgression?

The nuclear data inferred Hedysarum as a non-monophyletic lineage, whereas plastid sequences supported it as a monophyletic group. One of the reasons for this is chloroplast capture through...

25 January 2021 8,559 3 View