How to separate host DNA from microbial DNA?

Hello People I am extracting host DNA from fecal samples. However, feces contains lot of microbes and their DNA too gets extracted during DNA extraction procedure. I want to get rid/minimise...

05 June 2019 6,544 6 View

Unable to download r8s from its website for divergence time estimate.

02 March 2016 9,891 1 View

What is Nuclear Swamping?

Recently, I read about Nuclear Swamping in many articles. But, I don't think I completely understand what exactly is nuclear swamping. So if anyone has an explanation easier to understand, then...

11 December 2015 6,173 0 View

How can I import/open a nexus (*.nxs) file into MEGA 5.2?

I have nexus file (*.nxs) which contains a lot of taxa and the sequence length is also very long, so I cannot keep copy pasting it as it will take a lot of time. I want to know if there is any...

05 June 2014 5,766 6 View

Why to study phylogenetics? What's its use?

I work on the phylogeny of one of the mammal groups but sometimes I wonder, why exactly am I doing this? What's the point in studying phylogenetics? Is there any direct benefits of my study for...

31 December 2013 9,762 0 View

What is the best way for calculating phylogenetic diversity?

I need to calculate phylogenetic diversity (PD) of many different areas. I found some ways to calculate it, but couldn't decide on which way is better. If anybody has any idea about it please let...

09 October 2013 2,861 6 View

Which Taq should be used while working with DNA extracted from fecal samples?

I am trying to amplify mitochondrial as well as nuclear DNA from fecal samples but the success rate is very, very low. The reactions are failing miserably. I would like to know what kind of Taq...

07 August 2013 4,034 6 View

Why use a non-coding nuclear marker in phylogenetic and evolutionary studies?

Whats the difference when we use a coding nuclear marker versus the non-coding nuclear marker? What difference does it make to the results? And how do we know when to use a nuclear coding region...

05 June 2013 4,648 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View