How to separate host DNA from microbial DNA?

Hello People I am extracting host DNA from fecal samples. However, feces contains lot of microbes and their DNA too gets extracted during DNA extraction procedure. I want to get rid/minimise...

05 June 2019 6,544 6 View

Unable to download r8s from its website for divergence time estimate.

02 March 2016 9,891 1 View

What is Nuclear Swamping?

Recently, I read about Nuclear Swamping in many articles. But, I don't think I completely understand what exactly is nuclear swamping. So if anyone has an explanation easier to understand, then...

11 December 2015 6,173 0 View

How to convert excel format into mega format?

So is it possible to convert an excel file into mega format so that it can be opened in MEGA for alignment and analysis?

07 August 2014 1,290 3 View

How can I import/open a nexus (*.nxs) file into MEGA 5.2?

I have nexus file (*.nxs) which contains a lot of taxa and the sequence length is also very long, so I cannot keep copy pasting it as it will take a lot of time. I want to know if there is any...

05 June 2014 5,766 6 View

Why to study phylogenetics? What's its use?

I work on the phylogeny of one of the mammal groups but sometimes I wonder, why exactly am I doing this? What's the point in studying phylogenetics? Is there any direct benefits of my study for...

31 December 2013 9,762 0 View

What is the best way for calculating phylogenetic diversity?

I need to calculate phylogenetic diversity (PD) of many different areas. I found some ways to calculate it, but couldn't decide on which way is better. If anybody has any idea about it please let...

09 October 2013 2,861 6 View

Which Taq should be used while working with DNA extracted from fecal samples?

I am trying to amplify mitochondrial as well as nuclear DNA from fecal samples but the success rate is very, very low. The reactions are failing miserably. I would like to know what kind of Taq...

07 August 2013 4,034 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View