How to separate host DNA from microbial DNA?

Hello People I am extracting host DNA from fecal samples. However, feces contains lot of microbes and their DNA too gets extracted during DNA extraction procedure. I want to get rid/minimise...

05 June 2019 6,544 6 View

Unable to download r8s from its website for divergence time estimate.

02 March 2016 9,891 1 View

What is Nuclear Swamping?

Recently, I read about Nuclear Swamping in many articles. But, I don't think I completely understand what exactly is nuclear swamping. So if anyone has an explanation easier to understand, then...

11 December 2015 6,173 0 View

How to convert excel format into mega format?

So is it possible to convert an excel file into mega format so that it can be opened in MEGA for alignment and analysis?

07 August 2014 1,290 3 View

How can I import/open a nexus (*.nxs) file into MEGA 5.2?

I have nexus file (*.nxs) which contains a lot of taxa and the sequence length is also very long, so I cannot keep copy pasting it as it will take a lot of time. I want to know if there is any...

05 June 2014 5,766 6 View

What is the best way for calculating phylogenetic diversity?

I need to calculate phylogenetic diversity (PD) of many different areas. I found some ways to calculate it, but couldn't decide on which way is better. If anybody has any idea about it please let...

09 October 2013 2,861 6 View

Which Taq should be used while working with DNA extracted from fecal samples?

I am trying to amplify mitochondrial as well as nuclear DNA from fecal samples but the success rate is very, very low. The reactions are failing miserably. I would like to know what kind of Taq...

07 August 2013 4,034 6 View

Why use a non-coding nuclear marker in phylogenetic and evolutionary studies?

Whats the difference when we use a coding nuclear marker versus the non-coding nuclear marker? What difference does it make to the results? And how do we know when to use a nuclear coding region...

05 June 2013 4,648 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View