I have generated DC from mice bone marrow. I have followed very known protocol, incubating with GM-CSF for 8 days and LPS activation overnight or 48 hours.
After LPS activation, I stained my cells with CD11b, MHC II and CD 11C and then FACS analysing to be sure about DC population.
Results: I have around 30% CD 11b low, and 70% cd 11b high.
From gated CD 11b hi cells --> 70 % MHC II hi CD11 C + (activated DC) while 30% are MHC II hi CD11 -/low.
So I am wondering what are those CD11C low and MHC hi cells? Are they inflammatory monocytes (M1)? Or they are the precursor of CD11 hi cells?