What is meant by "The FC regaent was pre-diluted, 10 times with distilled water''?

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Is there any Impact of Interest Rate, Corruption Perception Index, and Economic Growth on Foreign Direct Investment in Developing Countries?

Is there any impact of the interest rate, corruption perception index, and economic growth on foreign direct investment in developing countries?

07 July 2024 770 7 View

Why is my TE-eluted cloned plasmid (isolated via miniprep) not freezing at -20 degree celsius? Is it normal?

I isolated my cloned plasmid from TOP10 cells using invitrogen plasmid miniprep kit and eluted it in TE buffer. But I noticed that contrary to my plasmid aliquot, it does not freeze at -20 degree...

19 June 2024 1,612 8 View

What is the basic difference between DTG and DSC in thermogravimetric analysis?

Please answer in context of cementitious composites. Is both are same? as from their analysis/graph I seen similar peaks at approximately similar temperature.

03 June 2024 7,447 5 View

In what specific applications are rotating heat pipes most advantageous compared to traditional heat pipes?

Explain your ideas

07 May 2024 4,553 2 View

What is the newest development in a pharmacy world, whether it's in human medicine or veterinary medicine?

I'm interested in developing new drug in veterinary medicine by changing the molecular aspect or find a new alternative treatment to some advance disease that's been happening in the world right...

06 May 2024 4,646 5 View

Can PVA be used as an additive in Aluminum Powder for achieving higher Sinter density?

I am an undergrad student and I need to suggest optimum amount of PVA to be added in Aluminum powder for achieving improved sinter density. Your guidance will mean a lot to me. Thanking in...

25 April 2024 4,892 0 View

Methylation, the best selection to get the real result when is reading the percentage of methylation, a colleague could help me with this question?

I have a important question to analyze the result of methylation percentage, but when we analyze the methylation result we focus in one DNA strand out of the two strands it has, however, we want...

19 April 2024 6,248 3 View

How can I get more cloud storage space on my devices, knowing that I have a university email, but my university only gives 100GB?

I don’t have enough space.

27 March 2024 9,707 0 View

How to confirm the homogeneous dispersion of fibres throughout the cement matrix?

04 March 2024 1,485 3 View

How can I re-concentrate my protein sample?

I have accidently added ripa in the cytosolic protein. How can I re-concentrate my protein sample? I was fractioning the mammalian nuclear and cytosolic proteins.

18 February 2024 5,073 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View