Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

How to remove straight line from mosaic Landsat image?

I have used mosaic to new raster tool to join two aerial image from Landsat 8 of different path. However, raster files had color variation and after mosaic I got a straight line running from...

22 December 2020 4,640 4 View

Can anyone please give me some good suggestions for my undergraduate mechanical engineering thesis?

Our group need to start giving topics on which one should we focus on regarding non renewable resources. I've looking these past 2months but nothing comes to mind.

27 November 2020 4,414 3 View

Can someone help with variable selection for logistic regression while considering missing data?

I have a data set with quite a lot of missing values, more in some variables than others. I performed bivariate analyses and I want to select variables for multivariate analysis. I understand that...

25 October 2020 3,613 3 View

To find maximum compressive stress in concrete using hognested model numerical?

In a reinforced concrete beam depth id 640mm.Depth of neutral axis is 0.43363d.Determine max compressive stress in concrete using hognested model when strain in steel reaches0.00284.Solve

20 October 2020 3,303 2 View

To simulate handover in wireless ad hoc networks, what are the freely available academic network simulation tools?

I wanted to simulate a handover algorithm for ad hoc networks, I want to know what are freely available supporting tools for academic research?

28 September 2020 4,604 7 View

What is the chemical name of Zn(0.3) Mg(0.7) Cr(x) Fe(2-x) O(4)?

Is this ferrite is from the spinel group? can anyone explain the tetrahedral and octahedral sites pf elements for this above formula? If Cr(2+) and if Cr(3+) is doped, describe the...

08 September 2020 4,472 2 View

What analysis will be best suited to analyze my likert type questionnaire with both likert item and likert scale type Data in SPSS?

https://forms.gle/METKBvvq6n6eFMLb6 Above is a link to my questionnaire. It has three objectives to evaluate. 1) From Page 2-6 the questionnaire ask the stress factors that effects the...

05 September 2020 3,960 3 View

What is the most efficient way to make quad meshes in 3D in ANSYS Fluent?

I am planning to run ANSYS Fluent simulations in 3D. While I have a lot of experience in meshing in 2D, I have no idea on how to form 3D meshes in ANSYS Fluent. The geometry I am working with has...

25 August 2020 7,902 3 View

How long time should we wait for editor decision on a manuscript?

Some editors keep a paper for a long time, more than 6 months or a year, without a decision. So I ask how a paper takes a long time like that for the review process and What the best way to...

13 August 2020 5,797 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View