Farhan Haider

11 Questions 14 Answers 0 Followers

Questions related from Farhan Haider

How to mine the TCGA Database for RNA sequencing?

Anyone with advice on how to mine data from TCGA Database. I am interested in RNA seq data, i have a gene of interest i to analyze but i cannot seem to locate where to indicate the gene i am...

14 November 2019 5,404 3 View

Does any one have Experience of PEI transfection with Gastric cancer cell-lines?

Hello, I am working on gastric cancer cell line, i try to transfected the gastric cell lines (SGC-7901, AGS, BGC823) according to standard protocol, but unfortunately they kill my cells, does...

21 May 2019 7,164 3 View

Suggest a relevent mouse model for HepG2 cell line?

suggest a relevent mouse model for HepG2 cell line, i am studying gene expression in tumorgensis. our desire gene show engough results in invitro studies, now i want to know about gene function in...

23 November 2017 5,978 5 View

How might one get uniform bands by western blot?

How can I get uniform bands by western blot? I try so many times with the same sample but I didn't get uniform bands (even for beta actin too).. I check the calculations for protein concentration...

07 November 2014 7,763 6 View

Why there are no clear bands when I run a Western blot for the GADD45a gene?

I ran a Western blot a few times for the GADD45a gene, but I didn't get clear bands, even when I changed the following conditions: High voltage (100V) to low voltage (80V) Gel concentration from...

16 October 2014 2,499 11 View

Can anyone help with a DPPH assay?

I use 1 ml of plant extract of different solvent (chloroform, hexane and methanol), and I prepare 5.9 mg of DPPH in 100 ml of methanol and check absorbance at 517nm. So please suggest how I can...

18 December 2013 7,421 3 View

What is meant by "The FC regaent was pre-diluted, 10 times with distilled water''?

Actually, I am going to perform determination of total phenolics content? I am confused as to whether they are asking for serial dilution or some thing else.

17 September 2013 6,558 3 View

How can I take the data from tissue cultured plants?

I want to take data from tissue cultured plant but I am confused how and from where I should start.

19 June 2013 7,231 8 View

hello i need MS Biotechnology scholarship or any professor or institute have research project i want to avail the project as MS Biotechnology student.

any professor or institute have research project in any country i am interested to join that research project as MS Biotechnology student...any person have project will accommodate me as student...

04 December 2011 3,571 2 View

biofuel production

any body who conduct research on biofuel plz help me...Malaysian government giving me scholarship and also give some topic to prepare proposal on them...i have no experience in making of proposal...

30 June 2011 5,574 2 View

biofuel production

any body who conduct research on biofuel plz help me...Malaysian government giving me scholarship and also give some topic to prepare proposal on them...i have no experience in making of proposal...

30 June 2011 5,907 1 View