Is it typical for phenol red to fade over time during cell culture?

I am incubating cells in RPMI in a CO2 incubator at 37C and 80% RH. The phenol red started as a pink color and after 24 hours in the incubator it has faded. The color is more or less the same,...

06 July 2019 7,428 4 View

What could cause high cytokine expression in untreated PBMC cell culture?

I am treated PBMCs with various compound to observe cytokine expression using ELISA, however, I am getting high expression in my untreated control cells as compared to my media-only control. I am...

05 June 2019 4,266 14 View

Why does the collection of cell culture supernatants need to be performed at 4C?

I am using cell culture supernatants in an ELISA. The cells are incubated at 37C, centrifuged, then the supernatants are used in an ELISA at RT. Why does the supernatant collection need to be at...

05 June 2019 914 4 View

Can anyone recommend a good marker for lymphocyte activation?

I am screening products against PBMCs, testing for potential immune activation. I have been looking at CD69 as a good general marker of immune activity, but would prefer something secreted. Does...

01 February 2019 2,285 7 View

How to best perform an ELISA to quantify cell surface antigens?

I would like to quantify the activation of cell surface antigens, in this case CD69, on peripheral blood mononuclear cells (PBMCs) to screen for an immune response. Do I need to take any special...

11 December 2018 3,657 6 View

Are water pans sufficient to maintain humidification of a CO2 incubator for cell culture?

I am trying to purchase a CO2 incubator to work with PBMCs. Are incubators with a water pan sufficient to keep the incubator humidified or is it worth it to pay for humidification control? The...

11 December 2018 3,347 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How to obtain free lipids starting from concentration over the CMC?

I need to work with free lipids at increasing concentration (50%, 60% of the CMC ect.). I'm now working with POPC and POPG. To reach these concentrations I have to start from concentration over...

17 February 2021 1,676 3 View