Natasha Worden

7 Questions 8 Answers 0 Followers

Questions related from Natasha Worden

Is it typical for phenol red to fade over time during cell culture?

I am incubating cells in RPMI in a CO2 incubator at 37C and 80% RH. The phenol red started as a pink color and after 24 hours in the incubator it has faded. The color is more or less the same,...

07 July 2019 7,530 4 View

Why does the collection of cell culture supernatants need to be performed at 4C?

I am using cell culture supernatants in an ELISA. The cells are incubated at 37C, centrifuged, then the supernatants are used in an ELISA at RT. Why does the supernatant collection need to be at...

06 June 2019 1,033 4 View

What could cause high cytokine expression in untreated PBMC cell culture?

I am treated PBMCs with various compound to observe cytokine expression using ELISA, however, I am getting high expression in my untreated control cells as compared to my media-only control. I am...

06 June 2019 4,474 14 View

What is insoluble in chlorform methanol solution?

I incubated plant material grindate in chloroform:methanol 2:1solution then filtered it to remove the lipid layer. What is left in the insoluble material? Carbohydrates and protein? and is...

05 May 2019 2,688 2 View

Can anyone recommend a good marker for lymphocyte activation?

I am screening products against PBMCs, testing for potential immune activation. I have been looking at CD69 as a good general marker of immune activity, but would prefer something secreted. Does...

02 February 2019 2,355 7 View

How to best perform an ELISA to quantify cell surface antigens?

I would like to quantify the activation of cell surface antigens, in this case CD69, on peripheral blood mononuclear cells (PBMCs) to screen for an immune response. Do I need to take any special...

12 December 2018 3,720 6 View

Are water pans sufficient to maintain humidification of a CO2 incubator for cell culture?

I am trying to purchase a CO2 incubator to work with PBMCs. Are incubators with a water pan sufficient to keep the incubator humidified or is it worth it to pay for humidification control? The...

12 December 2018 3,412 5 View