In your experience, what small indel, produced for gene knockout is easier to genotype by conventional multiplex PCR? In terms of easier assay design and robustness of further usage.
My master's thesis deals with the use of hot-tapping in pipeline repair. For my master's graduation work I really need references to regulatory documents and various international standards...
10 January 2021 6,815 3 View
Manufacturers and protocols recommend using Methanol, but in our lab they use Ethanol instead. Is there any difference? I need to stain PVDF membrane.
04 September 2020 9,855 3 View
Images from classic Hodgkin lymphoma, H&E, CD15, CD30, PAX5
11 December 2019 1,494 2 View
Dear Researchers, I have little problem, i want to know only this kind of problem with me, or also other researcher face same issue I am preparing CsPbI2Br perovskite with precursor CsI+PbI2+PbBr2...
05 July 2019 5,695 7 View
2X RNA Loading and ladder? I have to dilute 2X RNA loading dye with water. Usually I use 3 micro liter of loading with 3 micro liter of my sample. How much water should I use for diluting 2X RNA...
27 November 2011 520 3 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View