In your experience, what small indel, produced for gene knockout is easier to genotype by conventional multiplex PCR? In terms of easier assay design and robustness of further usage.
I have a case in my field research I want to know the consistency of the fish troops at Market X I recorded data for 7 days (from August 11 to August 17) regarding: (A) Fish sold by traders at...
27 September 2023 9,526 0 View
Saya mempunya sebuah kasus dalam penelitian lapangan saya Saya ingin mengetahui konsistensi pasukan ikan di Pasar X Saya mendata selama 7 hari (dari tanggal 11 Agustus hingga tanggal 17 Agustus)...
27 September 2023 9,295 0 View
Hello! I use a Sony SH 800 sorter to sort differentiated tumor cells (CD44-CD24+) of the T47D line. According to our and literature data, the CD44-CD24+ population in the T47D line is 70-80%,...
04 December 2022 988 2 View
My master's thesis deals with the use of hot-tapping in pipeline repair. For my master's graduation work I really need references to regulatory documents and various international standards...
10 January 2021 7,053 3 View
Manufacturers and protocols recommend using Methanol, but in our lab they use Ethanol instead. Is there any difference? I need to stain PVDF membrane.
04 September 2020 10,007 3 View
I need statistics on "Accidents at hazardous production facilities (gas pipelines, oil pipelines, boiler houses, coal mines and other enterprises that use hazardous substances)", and also as...
30 April 2020 7,470 0 View
I'm currently reading papers about in vivo retina activity measurement with calcium indicators and I can not understand, why the retinal photoreceptor cells do not activate in responce to the...
06 April 2020 7,771 0 View
Images from classic Hodgkin lymphoma, H&E, CD15, CD30, PAX5
11 December 2019 1,609 2 View
Dear Researchers, I have little problem, i want to know only this kind of problem with me, or also other researcher face same issue I am preparing CsPbI2Br perovskite with precursor CsI+PbI2+PbBr2...
05 July 2019 5,815 7 View
I am working on perovskite solar cell, i want to prepare 2D perovskite layer by adding Pb(SCN)2 in active layer
07 July 2018 2,525 9 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View