How to deal with one sided data set when using a naive bayes classifier?

Hello, I have built a naive bayes classifier in java in order to help sort through publications for a meta analysis project. When developing the classifier I used an available training set that...

09 June 2020 2,285 3 View

Do you know any undergraduates who published biomechanics research?

Have you, or anybody you can recommend, published applied biomechanics research conducted at least in part as an undergraduate? If so, please get in touch or recommend others. I'm asking for 5...

25 January 2020 7,649 5 View

What might cause photoresist pitting in an argon ion mill?

We are using Shipley 1813 as a mask for our process. The mill is an AJA argon ion mill running at 500 V, 56 mA. The resist seems to hold up well for a 10 minute etch, but by 40 minutes it is badly...

29 September 2019 9,256 1 View

Is it possible using Microsoft Word to compile an index for a book/document?

Book Mss completes in Word and publisher has asked for an index

12 June 2019 1,202 4 View

Recommended books on plant breeding and genetics?

Hi, I'm new here so sorry If I'm in the wrong place. I've been working in UK Agriculture for around 10 years now and I've decided to pursue a career in R&D and plant breeding after working...

30 March 2018 2,338 21 View

Is this a quantized formulation of General Relativity?

I have been working on a model of quantum gravity in which GR is formulated in the language of Quantum Mechanics .The latest version can be downloaded from the link below. Kindly peruse through to...

05 September 2017 4,074 9 View

What other internal controller can I use apart from the beta actin?

I carried out a real-time pcr for the expression of leucanthocyanidin reductase gene and on relative quantification I used beta-actin as my house keeping gene but it shows some changes in the...

08 October 2016 4,624 6 View

What are people's opinions of research on anthrogenic Carbon Emissions and their contribution to Global Warming?

Specifically how reliable is the science? It seems to me the empirical evidence is nowhere near what is required for cause & effect making this a statistical study of trends and potential causes.

09 June 2014 3,384 50 View

Can anyone help with the equipment for transporting brain slices or a section of oxygenated brain tissue?

I need to transport "live" human hippocampal brain tissue for use in electrophysiology and 2P imaging experiments. This is taken from a surgical suite in the hospital to our laboratory, which is...

23 February 2014 4,060 10 View

How can I identify introns and exons in a gene sequence( ORF)?

Which tools can I use to show these regions?

02 February 2014 1,046 15 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Can the problems of overfitting be solved without cross validation?

For an overfit data set the cross validation error will be much bigger than the training error. Is it true or false?

23 February 2021 3,616 2 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

How to inerpret whether PCR went OK?

Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...

14 February 2021 3,129 8 View