For presentations that prepared using web photos, who should be acknowledged ?

01 February 2017 4,332 2 View

Is there a study on the nasolacrimal duct of moose Alces Alces and saiga antelope?

31 December 2016 5,033 0 View

Fate map of epiblast in mice?

07 August 2016 8,523 1 View

What is better for decalcification of fish bones?

07 August 2016 8,545 5 View

What is the best method to stop hair shedding after stress?

Hair shedding and stress in women: what is the treatment?

06 July 2016 9,006 0 View

Chemical sclerosis or endo-vascular chemoablation (Sclerotherapy): do it work in arteries?

Is it possible to induce endo-arterial chemoablation?

06 July 2016 6,347 1 View

What is the best staining protocol for nerves in paraffin sections using silver nitrate?

Silver nitrate, accurate procedure for skin nervous elements (paraffin sections).

06 July 2016 1,270 0 View

What is bio-sonar organ ?

06 July 2016 6,053 1 View

What are the functions of the fontanelles in catfishes?

05 June 2016 1,168 4 View

What is the best method to stain nervous tissue in paraffin sections?

Do Silver nitrate is still recommended for staining nervous elements in paraffin sections? or there are more simple and accurate methods?

05 June 2016 9,373 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View