Do you have any advice for Cas9 purification?

I am thinking about purifying some Cas9 and was wondering if anyone had any tips/suggestions before I start doing some in-depth research. Some specific questions: Tagged vs untagged? (does the tag...

05 June 2015 5,466 3 View

How can one make a more stable mRNA design?

10 November 2014 3,827 4 View

Which tissues/organs have high levels of resident macrophages?

I know that macrophages reside in most tissues/organs, but I am wondering if anyone is aware of tissues or organs that have relatively high levels of macrophages (in healthy tissue, not during...

08 September 2014 8,116 25 View

Can anyone help me with immunoprecipitation from plasma?

We are interested in pulling a protein out of mouse plasma and then analyzing by western blot. We have a good sheep antibody that has been used for IP in the past, but not from plasma. We were...

02 March 2014 6,816 8 View

Do FLOW isotype controls really need to be fluorochrome matched?

I know that when doing FLOW it is important to include isotype controls of the same antibody subclass (e.g. IgG1) in order to make sure that your antibody signal is specific and is not being...

02 March 2014 6,622 25 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View