What is best method for isolation bacteriophages, for Bacillus?

28 May 2025 1 4K Report

Recently, The isolation of bacteriophages from sewage water is so difficult, How to obtain phages plaques when i make isolation bacteriophage experiments for these bacterial types as bellow;

1- Psychrobacter alimentarius.

2- Bacillus tropicus.

3- Leuconostoc mesenteroides.

and which better for use, (LB), or (MRS) Media?

Phil Geis

It's not hard for enteric bacterial phages.

Would you expect those species (and respective phages) to be present in sewage?

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