What is best extraction phase and mobile phase to be used as in lc-ms Gc-ms to identify antibiotics produced by actinomycetes species found in broth?

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Sawsan Abdalrahim

I need the best chemicals or solvents that inhance excellent yield of antibiotic

Frank Höger

Hi Sawsan

The extraction might depend a little on the properties of the expected molecules.

Do you expect your ABs as dissolved molecules in water or more in hydrophobic form, attached to the Biomass? Do you have ionic groups, basic, acidic or ampholytic?