What is B27 + Vitamin A alternatives for Brain Organoids?

27 March 2024 1 2K Report

Hello,

I am generating brain organoids. Some media require vitamin B27 +A, but we currently have vitamin B27 minus A vitamin. How bad will it be if we use B27 minus A instead of B27 + vitamin A? We also have retinoic acid. Would it be appropriate if B27 minus A were added (and how much)?

Adekoya Foluso

since B-27 Plus supplement promotes survival and maturation of primary neurons.

B-27 Supplement Minus is identical to B-27 Supplement except for the removal of antioxidant components.

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Burak Kahveci's questions See All

About forging of Mo Alloys ?

Hello dear Academicians, I am working on the forging of Mo alloys, but I wanted to consult you on an issue I could not solve. Mo is easily oxidized during forging and MoO3 is formed. Is it...

09 February 2021 1,989 3 View

Global change in protein expression?

Dear all, When I run my yeast total protein lysate extracted after 10 minute-treatment with my compound, I observe a global up regulation in proteins, even my loading control protein. I suspect...

03 January 2021 1,388 8 View

Molydenum Alloys Forging Process ?

Hello dear Academicians, I've been struggling on a specific subject for a while. Hence, I'd like to get some advice. I am working on Mo-0.5 Ti alloy production via powder metallurgy technic. I...

20 December 2020 3,805 2 View

Tetracycline/doxcycline treatment?

Hi all, I am not sure whether or not this question was asked. I wonder can I induce expression of my protein with tetracycline or doxycyxline while seeding cells instead of one day after seeding...

05 November 2020 2,417 4 View

How to distribute Titanium in Molybdenum host material via PM?

Hello dear Researchers, I've been working on to produce Mo-Ti alloys via powder metallurgy method. Yet, I've come across a problem: I can not properly distribute Titanium in Molybdenum host. I'm...

10 August 2020 4,502 3 View

How to distribute Titanium in Molybdenum host material via PM?

Hello dear Researchers, I've been working on to produce Mo-Ti alloys via powder metallurgy method. Yet, I've come across a problem: I can not properly distribute Titanium in Molybdenum host. I'm...

10 August 2020 5,909 4 View

A web application using database systems that developed on a local computer can be used in real systems?

For my research, I have designed a web application to connect different DBMS(Oracle, MySQL, SqlServer and PostgreSQL) on my desktop computer. Web application can access tables of these dbms. The...

26 July 2020 2,581 3 View

What is the best sintering temperature and environments for Zn-Mg alloys?

Hi everyone; I want to sinter Zn-3Mg compacts produced by powder metallurgy. The sample dimensions are d: 20mm and l:10mm. Could you please suggest to me the environment and temperature and time...

11 June 2020 2,922 10 View

How to count objects on the image with deep learning algorithms?

I am studying the first deep learning project. I need to counting tubules and cells. How to do this? If you suggest a tutorial (or another thing) related to this subject, I would love...

29 April 2020 989 7 View

qRT-PCR melting curves are good for cDNA of overexpression cells but not for cDNA of parental cells. What could be the reason?

I am trying to find a good qRT-PCR primer set for a gene that has low expression in our cell lines. As you see in the attachment I have a very sharp one peak when I used cDNA from overexpression...

23 February 2020 2,474 5 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View