What is/are the best free database and methods for comparative metagenomic analysis (16s rRNA)?

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Abhijeet Singh

You just need to revisit the following

https://www.researchgate.net/post/Which_bioinformatic_tool_is_feasible_to_explain_16s_metagenomic_data

https://www.researchgate.net/post/What_are_the_suitable_software_to_analyze_the_raw_DNA_sequences_meta-genomic_data_for_microbial_diversity_analysis

Ahmad Ali

Abhijeet Singh thank you so much for recommending the following

Salma Mukhtar

For analysis of 16S rRNA based metagenomic data, RDP classifier (https://rdp.cme.msu.edu/classifier/classifier.jsp;jsessionid=6E1CE186327ADDFD8168B86A88E7885E.10.0.0.9) can be used, it is free and online available. You can also use QIIME (1 and 2) software for Pyrosequencing or Illumunia sequencing based data