Working on presentation about metagenomics and alternative approaches, what's approaches stands for here ?

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Among the various globally adopted mix design methods for self compacting concrete which is the most widely used in recent times?

Recent practise of self compacting concrete mix design.

28 October 2014 2,280 2 View

Is there any treatment methods available for plastic coarse aggregates for fire resistance?

Is there any treatment methods available for plastic aggregates (polyethylene) used in concrete for fire resistance? (example: like steel rods are coated to prevent it from corrosion)

01 August 2014 3,427 2 View

Can anyone help with a quantum physics/linear algebra question?

Assuming we're going by Schrodinger's equation and the standard wave function of psi and delta x. What would happen if a wave function collapsed?

15 June 2014 4,623 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

Could anybody provide insights the best tools to create a custom database for taxonomy assignment of metagenomic data?

I am interested in the different tools that can be used to create custom databases for targeted sequencing and how to trim the databases based on the amplicon size? Also, should custom databases...

02 December 2020 3,105 3 View

No host reference for metatranscriptomics?

One of the steps during the preprocessing of the data from metatranscriptomic analysis is to remove any host reads (host contamination) by comparing to host database. But what if there is no host...

30 November 2020 3,259 2 View

Does Shannon index represent largest diversity index when the individual belongs to different species and vice versa?

The shannon wiener index and Simpson indices include the measurement of community heterogeneity. Shannon wiener index bapplied information theory. The main measuring object of information theory...

22 October 2020 1,053 7 View

Is the first clean up necessary in Illumina MiSeq Library Preparation?

I'm trying to streamline my metagenomics protocols for my lab. I have previously followed the 16S protocol...

16 September 2020 5,234 6 View

How to use Vegan::capscale to calculate and plot distance based- redundancy analysis (db-RDA) and add correlation cut-offs in the results?

I have a dataset with relative abundances of Genomes against their COG-ids. It is a large data frame with 4214 rows (COG-ids) and 1313 columns...

13 September 2020 2,334 1 View

Regarding metagenomic contamination?

We have lab established culture setup of marine synechococcus PCC 7002 sp. and heterotrophic bacteria. Recently we did metagenomic analysis of it (sequencing was done by an outside reputed...

12 August 2020 187 11 View