I need to sterilize hydrogel (silk fibroin and tannic acid) in gel form, for cell experiment .i added 75% ethanol and use UV irradiation for sterilization but the hydrogel was broke due to low degradability. please suggest me appropriate method that can i follow it would be highly appreciate .
Benish Nawaz
For the experiment with cells, you need a hydrogel, that is, a gel with water. Therefore, it is correct to disinfect with ultraviolet. Select the necessary radiation power. The starting power can be disinfection of water in a swimming pool.
You may try to filter the silk fibroin solution and prepare the hydrogel in the biosafety cabinetry. The UV irradiation is also necessary.
UV does NOT sterilize. To the extent it has efficacy, it must directly - line-of-site - impact each microbial. However, It penetrates water poorly, does not impact bacterial and most fungal spores, some microbial species are characteristically resistant(e.g. Deinococcus radiodurans, black fungi) and treated cells can recover in dark and light repair.
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