What would be a suitable vacuum pump for a solid phase extraction manifold (VAC ELUT SPS 24, 10 X 75MM RACK (from Agilent).
Dear Keerthi,
there are many consolidated providers supplying vacuum pumps which are suitable for a traditional solid phase extraction. For example, in the past I have used Thermofisher and KNF Laboport vacuum pumps and both resulted almost satisfying, without any significant difference. This notwithstanding, prior to buy a pump, you need information on the vacuum level and the pump speed you need to apply to your manifold (Consider that these parameters depend strictly and specifically on SPE cartridges as well as the adoption of unsatisfying levels of vacuum may drastically hamper the extraction/purification efficiency). Generally a pump providing a 20 L min pump speed and a vacuum < 10 mBar should be enough. However, you need to definitively take into account the technical specifications of the cartridges.
Best regards,
Pierluigi
low tech solution- you can pull a vacuum by turning on a faucet with the proper fittling. Bernouli Principle
We have always used a water aspirator as mentioned in the comment above. Use of excessive vacuum can interfere with the cartridge efficiency to selectively elute the analyte of interest.
I prefer to use a Diaphragm pump for SPE. I share a pump with my roto vap that has a vacuum controller. this pump provides a high degree of control and do not have the contamination issues that can happen is you use a oil pump.
Sorry, may be it is too late. But we use water pomp and it works well. All we have to do is to control the vacuum with a manometer and having a valve to adjust the pressure when needed. Please note that we work in a controlled environment, GMP, and the whole process has been validated. Regards, A.
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