First of all cells will be cultured on monolayer and than will be transformed to suspension. What is a suitable culture medium for each phase? Does anyone have any protocol or tips to help me with this method?
The 10 X1 L should come as 10 individual packets of powdered medium. It's also available as prepared medium. We don't usually grow ours in suspension, but it shouldn't matter.
Yes, %10 FBS. Ham's F12 was specifically developed for growth of CHO-K1, however Rongqing has a good point. Those are good resources to check. We also use Normocin to protect against Mycoplasma contamination.
In FBS 10% and DMEM F-12 HAm available with sigma. the cHO cell grow very well and after monolayer u can make the suspension by using the EDTA and trpsin solution..
even u can check the medium invitrogen or other available locally