I want to calculate antioxidant activity using such enzymes. Need referenced answers so that i can get help of it, and can design protocol to determine such biomarkers. References will be highly appreciated.
If you only want to perform these assays in a particular experiment, I thing it is easier to use commercial kits. On the contrary, if you want to stablish the protocols in your lab, certainly exists several ways to perform them, and all of them work well. You only need to know what instruments can you use (plate reader, standard spectrophotometer, fluorometer (plate of standard...) because all procedures have their little tricks. You can know our procedures in these documents:
Sir i just need results, not interested in protocol development. What you suggests for better and authentic results??? Protocol development in lab or using commercial kits????
The quick, easy and perfectly validated is the use of commercial kits. The problems: They are little expensive. Two brands have the best kits: Biovision and Cayman. You can see their webpages for information of their differents kits/sizes/prices. In my country Biovision is little cheaper than Cayman, but I have used both brands and both are very good.
Espectrophotometric assay are economic, easy and well documented, I suggest the methods of Misra and Fridovich (1972). Radi et al. (1991) and Lei et al. (1995) for SOD, CAT and GPx.