Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

Can Virtual Servers (remote dekstop manager) run heavy ANSYS Fluent CFD simulation ?

I have a virtual machine. My host Pc specs are not that good thats why i went for virtual servers. so basically my host pc has 8gb of ram with nvidia quadro k2200 4Gb DDR5 GPU ,processor intel...

01 March 2021 8,415 1 View

What is the difference between PBE and PBEsol functional?

Hello, I am a 3rd year chemistry student and I'm writing my project report on computational chemistry. does anyone know how PBEsol improves upon the PBE functional? Thanks in advance

19 February 2021 7,068 4 View

Can someone help me with this error that I am getting while estimating a VAR model in R?

I am using annual time series data to estimate a VAR model in R. However, when I run the command to find the optimal lag length, I get the following error: Fehler in VARselect(data, lag.max = 10,...

18 February 2021 9,647 3 View

In your experience what is the difference between G.652D and G.655D optical fiber. In field and in lab?

In our current era there is a big confusion about the usage of G.652 and G.655 optical fiber cable. Theoretically G.655 is much better than G.652 but the operator feel that G.652 is giving almost...

11 February 2021 4,006 8 View

How many dilutions are good enough for TCOD50 of SARS-CoV-2?

I started with 10e-1 till 10e-10. But when I searched for online calculator they mostly consider 5 dilutions. Is there a reason behind it or I should make less dilutions?

11 February 2021 5,839 3 View

Protocol for bacterial samples preparation for Scanning Electron Microscopy ?

I want to prepare bacterial samples to observe the bacterial cell lysis by bacteriophage protein (endolysin) through scanning electron microscopy. Please, anyone can share the protocol of...

01 February 2021 1,173 3 View

Which software to use to statistically analyze Telomere Length_TRF_Southern Blot?

I am trying to measure telomere length in an ant species using the TRF method, which is a Southern blot technique. Currently, I am struggling with analyzing the images and would appreciate any...

29 January 2021 604 3 View

Relative telomere length calculation with Ct values?

10 May 2020 7,398 2 View

How does telomerase affect telomere synthesis in chromosomes?

I understand telomerase is an enzyme that aids in telomere synthesis but i need a concise explanation

10 February 2020 6,370 3 View

How does telomerase affect telomere synthesis in chromosomes?

I understand telomerase is an enzyme that aids in telomere synthesis but i need a concise explanation

01 February 2020 2,861 3 View

What is the best protocol for Telomeric FISH?

I am working in telomere biology. We follow De Lange's protocol for Telomeric FISH. But unfortunately, the probe that is PNA does not bind with the telomeric repeats, though the metaphase...

08 September 2019 2,366 2 View

What is the easiest and cheapest way to quantify XY/XX ratio of cells in a mixed population of human cultured cell lines?

I have an XY cell line I am measuring telomere length on, and I am using an XX cell line (with abnormally long telomeres) as an internal control. I would like to identify each separately, but that...

26 August 2019 1,290 1 View

What is the easiest and cheapest way to quantify XY/XX ratio of cells in a mixed population of human cultured cell lines?

I have an XY cell line I am measuring telomere length on, and I am using an XX cell line (with abnormally long telomeres) as an internal control. I would like to identify each separately, but that...

07 August 2019 7,323 1 View

Does anyone have a good kit for telomerase activity and telomere lenght for rodent samples?

I have used TeloTAAGG from Roche in brain rodent samples but without good results. It seems that the restriction enzimes from the kit are not effective in these samples.

06 July 2019 7,362 2 View

Telomere length measurement by qPCR?

Hi everybody, I try to measure the telomere lenght by PCR method. First, I tried to use multiplex method. Now I try to measure them separately (telomere and single copy gene). But curves during...

06 July 2019 5,751 7 View

Telomere length measurement by qPCR?

Hi everybody, I try to measure the telomere lenght by PCR method. First, I tried to use multiplex method. Now I try to measure them separately (telomere and single copy gene). But curves during...

30 June 2019 4,269 7 View