Hello,
I am trying to visualize a genomic locus. I produced Alexa fluor 488 dUTP tagged FISH probes by nick translation. I used two PCR amplified DNA fragments (12kb and 16 kb) that span through 28 Kb region. Although PNA telomere probes work with no problem, my probes are giving high background signal. I used cot1 dna, hyblock dna, and salmon dna as blocking reagents. How can I fix this issue? I attached the pictures of telomeres and my probes
Rémi Neplaz
Hello,
By any chance, have you found a solution to the background signal issue with your FISH probe? It would be great to know if you've had any success with resolving this issue.
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