Any specific cell lines used to study telomere biology disorders?

More Sujitha Felicitus's questions See All

Why by convention kyphosis is a positive angle and lordosis is a negative angle?

By convention kyphosis is a positive angle and lordosis is a negative angle, with the patient facing to the viewer’s right side. How was that defined as positive and negative values?

09 March 2023 6,237 2 View

LCMS small molecules analysis?

I have a data generated by LCMS orbitrap Elite. The data is in .Raw format. How do I analyse this data. Can any one suggest me any commercial place who can help me in analysing the data? Is the...

11 October 2017 1,813 8 View

HLA typing. I am planning to start with HLA typing by SSP PCR before kidney transplantation. Any suggestions on how it is done in a diagnostic set up?

I am planning to start with HLA typing by SSP PCR before kidney transplantation. Any suggestions on how it is done in a diagnostic set up. Can I do it after Lymphocyte cross matching as a...

20 December 2015 2,349 5 View

Is venom activity ll active after the death of that venomous animal?

In venomous animals living conditions, venom glands are in function and hence its activity in releasing the venom causes lethal effects. After the death of the animal, venom in the animals shows...

06 September 2014 2,539 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,822 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,104 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,246 3 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Malcolm Nobre

Hello Sujitha Felicitus

You may use EBV-infected lymphoblastoid cell lines (LCLs) grown in RPMI Media 1640 supplemented with 20% FBS, penicillin and streptomycin, and 2 mM L-glutamine.

For more information you may refer to the article attached below. It will be helpful.

Article Poly(A)-specific ribonuclease deficiency impacts telomere bi...

Best.

Amos Bairoch

And searching for:

https://www.cellosaurus.org/search?input=Orphanet_1775

Gets you a list of 17 cell lines that have been established from DKC patients. Some of them are fibroblast, other LCL, iPSCs or even hESCs.