I was wondering if there is a general consensus as to which marker is more accurate and/or suitable at reflecting mast cell degranulation in vitro? I've read that beta hex is more accurate and histamine tends to be more inconsitently measured/detectable (more inconsistent results).
Looking forward to some experts' comments!
Thanks much
Hi,
Histamine and tryptase are the most specific for mast cells degranulation and they are preformed mediators! beta-hex also but it is not specific just for mast cells, because other cells also release beta-hex.
I hope that my answer helps you.
Mansour,
Thanks much! So beta hex is not preformed in mast cells? (in the granules?), is it produced once the cell is triggered? so that would be a different pathway right?
Thanks
Nat
Nat,
We usually use it as a marker of mast cell activation and degranulation, (it is more cheap that tryptase or histamine), we use in IgE and non-IgE activation and its work great.
Welcome
Mansour
Hi..
beta hex test has a margin of 5 % difference histamine test , and recalling that in the mast cell histamine is released not only at the time of degranulation , as there are other substances preformed and stored... dependes too the resources that you have... Good luck
Hello,
What is a more accurate marker of mast cell degranulation in vivo?
the histamine assay indicates the specific release of this substance, the beta hex allows you to have a general measurement of the mediators since the granules not only contain histamine but other mediators as well. You will have to evaluate which of the trials is more affordable in terms of cost/response.
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