Sabin Acharya

4 Questions 5 Answers 0 Followers

Questions related from Sabin Acharya

Is there any possible way or software for counting different cell count?

I am using photoshop to count each type of cells as in the picture and it is consuming a tremendous amount of time. I would really appreciate it if anyone let me know some kind of software that...

30 May 2021 4,450 4 View

Would you please be kind to share the possible ways to quantify the amount of mucus in the airway in the picture attached below?

I tried imageJ but ImageJ threshold option could not isolate the color of the airway only.

21 May 2020 9,073 2 View

What might be the reason for High OD of background in TNF alpha ELISA?

My protocol of ELISA is Capture Antibody coating (4 micrograms per ml) incubation overnight. Blocking (1% BSA in PBS) incubation for 1 hour. Sample loading (100 microliters of BAL fluid diluted in...

23 April 2020 3,873 3 View

What might be the reason for huge deviation in animal (mice) study during dosing of methacholine to measure lungs function test.?

I would like to let you know that I am doing an experiment in the asthma model mouse. To check the development of the diseases, I am using the flexivent machine. I am using the BALB/c mice and...

07 April 2020 4,281 0 View