I found a AGS cells sub-stock cryopreserved labelled as passage number 105. Is its passage number is too high? Is it possible to use it?
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View
> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’
18 July 2024 3,536 0 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...
04 July 2024 9,875 1 View
I synthesise intermetallic compounds through nickel and aluminium powders. But the pure powder after synthesis is difficult to obtain. Especially the presence of aluminium oxide very seriously...
01 July 2024 4,030 3 View
For the measurement of electrode reaction kinetics and electron transfer rate constant, using E. Laviron's model and RICHARD S. Nicholson's model, the greater peak potential separation indicates...
30 June 2024 580 3 View
Hi, I'm analyzing data from an oncology trial and trying to perform statistical analysis including cox regression analysis and paired t-tests. My data includes laboratory test values, including...
22 June 2024 6,181 1 View
"Nuclear-mitochondrial DNA segments (NUMTs) are mitochondrial DNA (mtDNA) fragments that have been inserted into the nuclear genome." (Xue et al., 2023) I would like to know under this...
20 June 2024 603 1 View
Hello. We have a sample with an unknown concentration of polyphenols. We're diluting the sample in order to perform the Folin Ciocalteau method. 0.2 grams sample / 4 mL 70% methanol and 30%...
10 June 2024 2,134 3 View
I preprared curcumin-loaded liposome by Thin Film Hydration Method using lecithin, curcumin and beta-sterol. I stained it with phosphotungstic acid and uranium acetate and took TEM images of each....
30 May 2024 6,863 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Can we preserve the isolated protoplasts for future experiments? Is it possible to preserve them via cryopreservation? If yes how long can we preserve them?
16 July 2024 9,576 1 View
Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...
08 July 2024 3,150 2 View
The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....
01 July 2024 3,183 0 View
Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...
19 June 2024 746 0 View
My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....
19 June 2024 2,680 1 View
During electrocatalytic nitrogen reduction to ammonia, we perform isotopic labelling test using HNMR to confirm source of ammonia.
29 May 2024 5,165 0 View