Lin Jin Zhi

4 Questions 5 Answers 0 Followers

Questions related from Lin Jin Zhi

Are the cells shown in attached JPEG really HepG2 cells? May I know your opinions?

I recently received a cell stock labelled as hepG2. But when I cultured it, its morphology showed as attached photo. I think the morphology is different from commercially available HepG2 cell...

14 May 2021 2,641 6 View

How much of protein do you usually obtain after protein lysis for western blot? How much is the proper concentration of protein?

I did protein sample preparation for western blot. The conditions are as followed; 90mmx20mm culture dish 500ul RIPA buffer ( containing aprotinin, leupeptin, pmsf, phosphatase inhibitor...

18 November 2019 5,579 4 View

How does acridine orange works to recognize apoptosis, autophagy or lysosomal stability?

I used acridine orange staining to detect autophagy. First, the cells were grown in RPMI 10%FBS in confocal dishes, treated with compound with or without with wortmannin (0.5uM). Wortmannin was...

09 August 2019 9,424 5 View

What should I do when drug-induced cell death is increased twice than before?

I'm now finding the pathway of cell death induced by drug in AGS cell lines. As my senior did cell viability assay to drug-treated cells, I didn't do it again and I use the data from her...

14 July 2018 6,531 6 View