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Questions related from Lin Jin Zhi
I recently received a cell stock labelled as hepG2. But when I cultured it, its morphology showed as attached photo. I think the morphology is different from commercially available HepG2 cell...
14 May 2021 2,655 6 View
I did protein sample preparation for western blot. The conditions are as followed; 90mmx20mm culture dish 500ul RIPA buffer ( containing aprotinin, leupeptin, pmsf, phosphatase inhibitor...
18 November 2019 5,591 4 View
I used acridine orange staining to detect autophagy. First, the cells were grown in RPMI 10%FBS in confocal dishes, treated with compound with or without with wortmannin (0.5uM). Wortmannin was...
09 August 2019 9,452 5 View
I found a AGS cells sub-stock cryopreserved labelled as passage number 105. Is its passage number is too high? Is it possible to use it?
29 October 2018 3,310 0 View
I'm now finding the pathway of cell death induced by drug in AGS cell lines. As my senior did cell viability assay to drug-treated cells, I didn't do it again and I use the data from her...
14 July 2018 6,548 6 View