How do I do multiple restarts is ABAQUS ?

Hello All, I have been trying to restart an ABAQUS simulation using the command line. The following works fine ######## abaqus job=restart1.inp oldjob=OLD user=UMAT.f cpus=160 scratch=$PWD...

25 March 2019 6,443 5 View

How can DNA extracted from Fossils be reliable data for determining evolution?

Due to age-old exposure to sunlight , pressure, temperature, and difference in pH, is it not possible that the DNA within the fossils would have been mutated? How can it be considered as reliable...

28 March 2018 5,016 4 View

In Abaqus, How do I pass the displacement information obtained at step -1 to Step-2 ?

I am carrying out a coupled thermal-mechanical simulation in Abaqus, using my own UMAT.At the first step, I am just running the thermal case with kinematic constraints.After this step, I obtain...

30 May 2017 8,506 3 View

Could I use C3D8R element instead of C3D8 to reduce simulation time in Abaqus ?

I am doing an FE simulation of a polycrystal ( uniaxial). I currently am using C3D8 elements to simulate the same. I would like to reduce the simulation time, Hence I am planning to use a C3D8R (...

18 April 2017 2,559 6 View

How do we intutively understand the elastic spin tensor (We) and plastic spin tensor (Wp) in the framework of crystal plasticity ?

07 August 2016 9,291 10 View

How do we define the total work energy density for a material undergoing plastic deformation ?

31 July 2016 4,249 3 View

Software for docking

Can anyone suggest me any free software for docking?

15 June 2012 5,486 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View