My iPS cells differentiate and do not maintain proper morphology on new MEFs that I currently use, so I would like to use another source. Does anyone have recommendations?
Hi, view
http://www.jove.com/video/3854/preparation-mouse-embryonic-fibroblast-cells-suitable-for-culturing
Hi, I would recommend using global stem CF1 irradiated or mitomycin C blocked, both are good. They have high viability and are US based. I have tried R&D wasn´t very impressed with them. Hope that helps
Hi, I use a commercially available MEFs for my human iPSC culture from GlobalStem company. I usually buy the CF-1 MEF (already irradiated or untreated and then I expand them and inactivated them with mitomycin) and it always works nicely. Good Luck
Here the link http://www.globalstem.com/feeder-cells/mouse-fibroblasts/cf-1-mef
Hi, I've got mine from ATCC =) http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2214&Template=cellBiology, though I inactivate them myself with mytomicin from Sigma. So far no problem =).
I have used ATCC ones successfully... nevertheless depending on the usage, it can be convenient and cheaper to prepare own batches...
it completely depends on your time and budget.If you have no time I recommend you to don't waste time by isolation and irritating process and order a vial of them.in my case,I ordered from iDNA company.
In our hands, Globalstem MEF works well; but some big labs prefer to make their own.
Life Line Cell Technologies. With their medium, their cells grow like crazy, and you can expand to 100's of vials!
That answer was incorrect, as I forgot to say these are human cells and not MEFS.
REF feeder cells are better than MEFs in supporting human iPSC generation and culture. REFs can be purchased from ABM.
It's so easy to make your own though! Just get a 16-d/o pregnant mouse and isolate the embryos. Crush them up and expand them in T-175 flasks for a few days. Then, freeze them at around 10 M cells/vial. They need to be primaries; so re-purchasing from a vendor can get expensive fast!
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