9 Questions 23 Answers 0 Followers
Questions related from Nasibeh Daneshvar
Can EBs be formed without growing them in adherent culture? I grew my EBs in suspension culture for 6 days and then as I saw their morphology under microscope, discarded them to extract their RNA...
17 October 2013 4,896 2 View
CFU assay
29 September 2013 1,349 0 View
I tried to culture MEF feeder layer cells using MEF media which contains: DMEM containing 10% FBS, 2mM glutamine, 1x10-4M nonessential amino acids, and 50 U and 50 mg /ml penicillin and...
03 May 2013 6,402 1 View
I thaw a vial of MEF feeder layer cells of irradiated mouse embryonic fibroblast (iMEF) feeder cells from CF1 mice (GlobalStem), and culture in high glucose DMEM supplemented with 10% serum,...
13 April 2013 7,322 20 View
One of our suppliers introduced me to Cultrex® BME PathClear and Geltrex® Matrix as the alternatives to matrigel. I was wondering as if anybody has experience of using these products?
06 March 2013 6,251 2 View
I need to do flow cytometry, I was wondering which brand has the best primary and conjucated secondary antibodies for a robust detection.
18 February 2013 4,652 8 View
I am trying to keep the induced cell colonies undifferentiated without using any feeder layer, but they seem to be differentiated, their morphology doesn't look like the normal ES cells. How can I...
06 February 2013 3,719 29 View
Media serum
23 January 2013 4,573 18 View
methods
23 January 2013 4,545 1 View
