Nasibeh Daneshvar

6 Questions 22 Answers 0 Followers

Questions related from Nasibeh Daneshvar

MEF medium

I tried to culture MEF feeder layer cells using MEF media which contains: DMEM containing 10% FBS, 2mM glutamine, 1x10-4M nonessential amino acids, and 50 U and 50 mg /ml penicillin and...

03 May 2013 6,373 1 View

Best media for MEF feeder layer cells

I thaw a vial of MEF feeder layer cells of irradiated mouse embryonic fibroblast (iMEF) feeder cells from CF1 mice (GlobalStem), and culture in high glucose DMEM supplemented with 10% serum,...

13 April 2013 7,272 20 View

Matrigel alternatives

One of our suppliers introduced me to Cultrex® BME PathClear and Geltrex® Matrix as the alternatives to matrigel. I was wondering as if anybody has experience of using these products?

06 March 2013 6,219 2 View

Which brand of antibody is best for using in flow cytometry and ICC?

I need to do flow cytometry, I was wondering which brand has the best primary and conjucated secondary antibodies for a robust detection.

18 February 2013 4,595 8 View

Ipsc colonies state

I am trying to keep the induced cell colonies undifferentiated without using any feeder layer, but they seem to be differentiated, their morphology doesn't look like the normal ES cells. How can I...

06 February 2013 3,672 29 View

What is the difference between KO Serum replacement and FBS, and which one is better for use in ipsc media?

Media serum

23 January 2013 4,535 18 View