Machine Learning and Big Data Analytics Tools Question?

What would be the best big data analytical tools to determine changes in behavioral health (e.g., addiction behaviors)? The data used to predict behavioral health changes are secondary data which...

30 September 2020 9,522 8 View

Why does my starting culture for maxiprep not able to grow?

I have my plasmid (pCMV-4 backbone) in a DH5alpha cells and I have spread it on a LB+AMP plates (final conc of AMP= 0.1mg/ml). These plates have colonies that grew well with the expected shape and...

16 August 2019 2,866 1 View

Recovered Carbon Black from Waste Tire Pyrolysis: How to estimate the level of odor of recovered carbon black (due to VOC)?

Dear All, Currently, we are facing quality issue of our recovered carbon black(rCB) that our customer complained our rCB got unpleasant smell. I suspected those are due to volatile matter...

05 August 2019 9,657 2 View

Can e-flows be estimated based on aquatic primary producers?

River ecosystem health or aquatic ecosystem health could be determined using Index of Biotic Integrity (IBI). E-flows describe the quantity, timing, and quality of water flows required to sustain...

27 February 2019 4,451 3 View

How to tweak the attached code to accept 3 user entry as input and produce 1 binary output?

I have trained a NN with nprtool in MATLAB. However, I am planning to deploy this code with 3 user entry as input and the code will generate 1 output in binary of 1 or 0. Humbly may I ask for...

18 October 2018 2,833 2 View

What are the ways to improve the accuracy of LAMP detection?

I am currently working on a project which uses LAMP to detect Bacillus cereus. The specificity test we did earlier has no problem with the No-Template Control, however when we move on to the...

11 October 2018 5,167 2 View

LAMP NTC showing amplification while the DNA concentration do not show amplification?

I am working on a school project and we are performing LAMP on Bacillus cereus. Currently we are working on the sensitivity of the LAMP. We have performed the experiment for 3 times and for all 3...

24 September 2018 6,032 2 View

Tailed dots in 2D gel electrophoresis after CBB staining?

I have recently run IEF (7cm, PH 3-11, NL) and 2D gel electrophoresis (12.5%). After CBB staining, the dots were not shown as single dots. Most of them are tailed (see attached). I would guess...

25 March 2018 8,477 3 View

Can Salmonella enteritidis express protein under lac promoter in a designed plasmid?

I am wondering if Salmonella enteritidis can transcribed a gene which is under lac promoter? Since most of the salmonella is lacking lac operon and I am not sure the specificity of the RNA...

15 June 2017 4,682 5 View

How to convert C++ to matlab m file?

19 April 2017 9,608 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View